[PDF][PDF] Knockdown of glucocorticoid receptor (GR) using a combined RNAi and Cre-LoxP system and promotion of GR expression by Youguiyin.
XY Li, JF Sun, XY Xu - European Review for Medical & …, 2012 - europeanreview.org
XY Li, JF Sun, XY Xu
European Review for Medical & Pharmacological Sciences, 2012•europeanreview.org… We aimed to establish one cell model whose the GR gene had been decreased and
observe the effect of the traditional Chinese medicine Youguiyin on GR gene expression at
mRNA level. MATERIALS AND METHODS: Established recombinant plasmids of GR gene
by combination use RNAi and the Cre-LoxP system, stably transfected the recombinant
plasmids and Cre-ERT2 plasmid into the murine macrophage RAW264.7 cells and selected
with G418 and hygromycin B respectively, then used 4-TH to induce Cre-ERT2 plasmid to …
observe the effect of the traditional Chinese medicine Youguiyin on GR gene expression at
mRNA level. MATERIALS AND METHODS: Established recombinant plasmids of GR gene
by combination use RNAi and the Cre-LoxP system, stably transfected the recombinant
plasmids and Cre-ERT2 plasmid into the murine macrophage RAW264.7 cells and selected
with G418 and hygromycin B respectively, then used 4-TH to induce Cre-ERT2 plasmid to …
CONCLUSIONS: Combination use RNAi and the Cre-LoxP system can decrease GR gene’s expression in the murine macrophage RAW264. 7 cells. Youguiyin can enhance the expression of GR mRNA.
europeanreview.org
以上显示的是最相近的搜索结果。 查看全部搜索结果